Secure Air Traffic Control at the Hub of Multiplexing on the Centrifugo-Pneumatic Lab-on-a-Disc Platform

Fluidic larger-scale integration (LSI) resides at the heart of comprehensive sample-to-answer automation and parallelization assay panels for frequent ubiquitous bioanalytical testing in decentralized point-of-use/point-of-care settings. This paper develops a novel “digital twin” strategy with an emphasis on rotational, centrifugo-pneumatic flow control. The underlying model systematically connects retention rates rotationally actuated valves as key element LSI to experimental input parameters; first time, concept band widths frequency space decisive quantity characterizing operational robustness is introduced, set quantitative performance metrics guiding algorithmic optimization disc layouts defined, engineering principles advanced, logical control timing are elucidated. Overall, digital twin enables efficient design automating multiplexed bioassay protocols such “Lab-on-a-Disc” (LoaD) systems featuring high packing density, reliability, configurability, modularity, manufacturability eventually minimize cost, risk development production.